Coding

Part:BBa_K2638100:Design

Designed by: Johannes Ruhnau   Group: iGEM18_Bielefeld-CeBiTec   (2018-08-31)


alkyl hydroperoxide reductase, C22 subunit (ahpC)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Removal of the restriction sites EcoRI and PstI by substitution of cytosine at position 63 with thymine and substitution of guanosine at position 186 with adenosine


Source

genome of Escherichia coli K-12 DH5alpha

References

Seaver, L. C., & Imlay, J. A. (2001). Alkyl hydroperoxide reductase is the primary scavenger of endogenous hydrogen peroxide in Escherichia coli. Journal of bacteriology, 183(24), 7173-7181.